Journal: Journal of neuroscience research
Article Title: Anxiety-Like Behaviors in Mice Unmasked: Revealing Sex Differences in Anxiety Using a Novel Light-Heat Conflict Test.
doi: 10.1002/jnr.70002
Figure Lengend Snippet: FIGURE 1 | The Thermal Increments Dark–Light (TIDAL) conflict test for exploring anxiety-like behavior and thermal sensitivity in mice: Design, controls, timeline, and predictions. (A) Final, optimized setup of the TIDAL apparatus. Note that the overhead room lights are on, and the dark-heated plate is covered with a lid and opaque film. (B) Setup of the Thermal Place Preference (TPP) apparatus, a control condition that removes the light–dark (anxiety-related) aspect of the TIDAL conflict test to isolate the effects of shifting heat on one plate. (C) TIDAL prototype setup for data described in Figures S1 and S2. Note lighting using focal lamps (rather than overhead lights) and lack of a lid on the top of the dark plate. (D) Optimized TIDAL/TPP test timeline. In TIDAL, the heat-shifting plate is dark and the isothermic plate is lighted, whereas in TPP both plates are lighted. Mice are placed in the apparatus with both sides set to 31°C. The first 5 min are recorded as an initial dark–light test, a control condition that reveals baseline preference for the dark (a measure of anxiety-like behavior). Mice remain in the apparatus as the heating plate continues for 5 min at 31°C, then 39°C, then increases incrementally 1°C every 5 min through 44°C. The lighted isothermic side is maintained throughout at 31°C. (E) Using the TIDAL conflict test, mice are expected to initially prefer the dark side, but will increasingly avoid the heated-dark plate as its temperature increases. We predict that sex and neu- rotrauma will alter the salience of heat discomfort versus anxiety-related sensitivity, manifesting as shifted heated-dark plate preferences over time.
Article Snippet: The TIDAL conflict apparatus is a modified thermal place preference (TPP) apparatus (Ugo Basile, Cat. No. 35250), which consists of two cylinders (20 cm diameter × 25 cm high) connected by a narrow center walkway (Figure 1).
Techniques: Control
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